Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal URCA FARE 3BCAR

Fractionation of AgroResources and Environment lab

Laboratoire d’Analyses d’Isotopes Stables (LADIS)

AEBB Grand Est – LADIS

UMR FARE offers a service of elementary and stable isotope analyses (LADIS) for researchers and engineers from Public Scientific and Technological Establishments (EPST), as well as applied Research Institutes and Higher Education Establishments identified in the field of Agricultural and Environmental Sciences (environmental biology, plant and microbial biology, agronomy, soil science).

Fields of application

The laboratory performs simultaneous quantification of stable isotopes of carbon (13C/12C), nitrogen (15N/14N) and sulphur (34S/32S) from natural abundance to a maximum isotopic excess of 10 %, on solid (soil, sediment, plants, fungi, macromolecules), liquid (drainage water, soil solution, plant extract) or gaseous (aliquot of atmosphere) samples. 

The laboratory has first-rate equipment acquired in 2019 and integrated into the regional platform AEBB (Agro-sciences Environnement Biotechnologies and Bioeconomy). It is based on scientific experience and technical know-how in the use and analysis of these stable isotopes.

Committed to quality assurance in research and connected to the European inter-laboratory analysis network WEPAL, through the international programme for the exchange of soil and plant samples, LADIS has the capacity to respond to diversified analytical demands with or without scientific collaboration. It is under the responsibility of two INRAE engineers.

30 years of experience

Created at the end of the 1970s at the INRA Agronomy station in Laon (Aisne Department), the laboratory has become over time a national service of the Environment and Agronomy Department (presently named AgroEcoSystem) of INRAE. Since 2008, it has been a component of UMR FARE, and is located at the Centre de Recherche en Environnement et Agronomie in Reims. The scientific topics addressed in the UMR involve the use of isotopes to study the degradation of lignocellulosic biomasses and the biogeochemical cycles of nitrogen and carbon in the soil/plant/atmosphere continuum.

Today, with more than 30 years of experience, LADIS provides this service of sample analysis, support and training in sample preparation for INRAE, CIRAD research units, as well as for French and foreign academic organizations, e.g., University of Marseille and Reims, ISARA Lyon, Terres Inovia, Swedish University of Agricultural Sciences, etc.

Isotopic Ratio Mass Spectrometers (IRMS) and their coupled peripheral modules 

Delta Plus Advantage (Thermo Electron, Bremen, Allemagne) : δ13C , δ15N

- Elemental Analyze C N, EA EuroVector (EuroVector, Pavia, Italie) : %N, %C

Eurovector

______

PrecisION (Isoprime, Cheadle Hulme, Royaume-Uni) : δ13C , δ15N , δ34S

Isoprime

- Elemental Analyzer C N S,  Vario isotope Cube (Elementar, Hanau, Allemagne) : %N, %C, %S,

- Dissolved Organic Carbon Analyzer  IsoToc Cube (Elementar, Hanau, Allemagne) : mgL-1 COD

CO dissout

- IsoFlow gas module  (Isoprime, Cheadle Hulme, Royaume-Uni) : Analyse CO32- , CO2 , N2

Isoflow

Contacts and analysis requests

Technical Centre Chair : G. Alavoine, gonzague.alavoine@inrae.fr

Technical manager : O. Delfosse, olivier.delfosse@inrae.fr

For further information and before requesting analyses, please contact Olivier Delfosse by e-mail.

Afterwards, the analysis request form below should be completed and returned

Our references (internal and partners publications 

Bertrand I., Delfosse O., Mary B. (2007) Carbon and nitrogen mineralization in acidic, limed and calcareous agricultural soils: Apparent and actual effects. Soil Biology and Biochemistry 39 276-288. DOI

Coppens F., Alavoine G., Delfosse O., Recous S. (2009) Evaluation of sample pre-treatment and potential contamination of soluble carbon in soil extracts during lyophilization  Soil Biology & Biochemistry, 41, 6, 1335-1337 DOI

Prache S., Kondjoyan N., Delfosse O., Chauveau-Duriot B., Andueza D., Cornu A. (2009) Discrimination of pasture-fed lambs from lambs fed dehydrated alfalfa indoors using different compounds measured in the fat, meat and plasma. Animal , 3, 598-605. DOI

Salon, C. ; Voisin, A.S. ; Delfosse, O. ; Mary, B. (2010) Methodologies for measuring symbiotic nitrogen fixation in the field. Munier-Jolain, N. (Coordinateur) ; Biarnès, V. (Coordinateur) ; Chaillet, I. (Coordinateur) ; Lecoeur, J. (Coordinateur) ; Jeuffroy, M.H. (Coordinateur). Physiology of the pea crop. Boca Raton (USA) : CRC Press ; 83-87.

Devincenzi T., Delfosse O., Andueza D., Nabinger C., Prache S. (2013) Dose-dependent response of nitrogen stable isotope ratio to proportion of legumes in diet to authenticate lamb meat produced from legume-rich pastures. Food Chemistry, 152 : 456-461. DOI

Attard E., Le Roux X., Charrier X., Delfosse O., Guillaumaud N., Lemaire G., Recous S. (2016) Delayed and asymmetric responses of soil C pools and N fluxes to grassland/cropland conversions. Soil Biology & Biochemistry, 97, 31-39. DOI

Autret B, Mary B, Chenu C, Balabane M, Girardin C, Bertrand M, Grandeau G, Beaudoin N (2016) Alternative arable cropping systems: A key to increase soil organic carbon storage? Results from a 16 year field experiment. Agriculture, Ecosystems & Environment, 232, 150-164, DOI

Ferchaud F., Vitte G., Machet J-M., Beaudoin N., Catterou M., Mary B. (2016) The fate of cumulative applications of 15N-labelled fertiliser in perennial and annual bioenergy crops, Agriculture, Ecosystems & Environment, 223, 76-86, DOI 

Lashermes G, Gainvors-Claisse A, Recous S, Bertrand I (2016) Enzymatic Strategies and Carbon Use Efficiency of a Litter-Decomposing Fungus Grown on Maize Leaves, Stems, and Roots. Frontiers in Microbiology 7:1315. DOI

Sauvadet M, Lashermes G, Alavoine G, Recous S, Chauvat M, Maron PA, Bertrand I (2018). High carbon use efficiency and low priming effect promote soil C stabilization under reduced tillage. Soil Biology and Biochemistry, 64–73. DOI.

Fanin, N. Alavoine, G. Bertrand, I. (2020), Temporal dynamics of litter quality, soil properties and microbial strategies as main drivers of the priming effect. Geoderma, 377, 114576. DOI