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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan cedex - France

Dernière mise à jour : Mai 2018

Menu Logo Principal Université de Lorraine

UMR EEF - Ecologie et Ecophysiologie Forestières

          www.nancy.inra.fr/eef

Didier Le Thiec

Physiologie et diversité de la réponse aux contraintes

Didier LE THIEC

Directeur de Recherches 2ème Classe INRA
UMR INRA-UHP " EEF : Forest Ecology and Ecophysiology" F 54280 CHAMPENOUX
(33) +3 83 39 40 98
didier.lethiec@inra.fr

Field of expertise :

  • Forest Tree Ecophysiology

Main areas of interest :

It is well established that stomata play a vital role in plant adaptation to abiotic stress conditions by opening and closing, Plants regulate their water loss by means of specialized guard cells. These pairs of cells surround the stomatal pore, and control the rate at which plants lose water (transpiration) by shrinking and swelling. When guard cells swell, the stomatal pore increases in size, allowing the plant to lose water. When water is limiting, as it is in many of the world's arid environments, the guard cells lose turgor. This ensures that the guard cells collapse around the stomatal pore, effectively blocking transpiration, and allowing the plant to conserve water. Stomatal regulation is crucial for the plants' survival in a constantly changing environment.. Understanding how plants respond to conditions of drought and ozone, is central to our hope that in the future we may be able to engineer plants to withstand severe lack of water, and thus allow trees to grow in places where they cannot presently survive.

Currently I have three major research projects in the lab. One is a study of processes (signal transduction, detoxification) in guard cells in response to ozone and water stress. The second is a molecular ecophysiology elucidation of the role of stomata in water use efficiency, using the model plant system, Populus. The third is a the replacement of the ozone-impact indices currently used at the European level (AOT40), that rely only on the cumulative external ozone concentrations, by more relevant indices based on stomatal aperture and plant defence mechanisms.

populus Populus

Techniques and Protocols :

Stomatal pores in the epidermis of leaves allow CO2 (and other gas like ozone) exchange with the atmosphere and account for transpirational water loss of plants.

coupe feuille  Leaf section
hydro

Water transfert

Two guard cells surround each pore and control the opening and closing of stomata. I am combining analyses of :

  • gas exchanges (with portable system). The gas exchange system studies stomatal opening and closing by examining the changes in the CO2 concentration and humidity of the air moving through the system.

divers-2

stoma Stomata Open - Stomata Close
  • new transcriptomic and metabolomic approaches towards discovering new signaling and responses mechanisms. Guard cells are collected by laser capture microdissection. Under a microscope using a software interface, a tissue section (typically 5-50 microns thick) is viewed and individual cells or clusters of cells are identified either manually or in semi-automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation. The laser capture microdissection process does not alter or damage the morphology and chemistry of the sample collected, nor the surrounding cells. For this reason, LCM is a useful method of collecting selected cells for RNA and/or metabolite -protein analyses

lcmmicrodissec

LCM - Microdissection

microdissection-1

Guard Cell Microdissection

microdissection-2

  • element content with Energy and Wavelength dispersive X-ray (EDX and WDX) spectrometers.

meb_web

Scanning electron microscope

  • stomatal density with SEM (controlled pressure). Many genes controlling Arabidopsis stomatal development have been identified providing a better understanding of how stomata form and are spaced in a fashion that maximizes gas diffusion. However, only a few studies have addressed the relationships between stomatal development and patterning, and their biological functions, especially under abiotic stress

sdpop-2(1)

sdpop-1(1)

sdpop-3(1)

Development of Guard Cell during leaf Ontogeny

Main actual project :

VULNOZ Project (ANR) Vulnerability to ozone in anthropized ecosystems. Which risks for 2020-2030 ?

In industrialized countries, ozone (O3) is an important gaseous air pollutant with phytotoxic effects due to strong oxidizing properties. Model projections using IPCC scenarios (2007) for the 21st century indicate that concentrations of ozone will by far exceed internationally accepted critical levels. Spatial and temporal variations can be expected with increased background concentrations from May to September and elevated frequency of summer peaks in peri-urban areas. An increased vulnerability of agroecosystems could occur.

The current models do not sufficiently take into account two essential aspects: (i) the division between stomatal and non-stomatal deposits, knowing that the former only affect plants and (ii) detoxification capacity of ozone and ozone-derived oxidizing molecules by plants. The partners in VULNOZ aim at improving knowledge on basic plant response mechanisms to ozone in order to integrate them in global ozone impact prediction models suitable for economic risk assessment. Results will allow the replacement of the ozone-impact indices currently used at the European level (AOT40), that rely only on the cumulative external ozone concentrations, by more relevant indices based on stomatal aperture and plant defence mechanisms.
wpvulnoz

Cooperation:

cooperation_1

cooperation_2

Publications:

Referee for Ambio, Annals of Botany, Annals of Forest Science, Journal of Experimental Botany, New Phytologist, Photosynthesis Research, Tree Physiology ….

  • WILDHAGEN H, PAUL S, ALLWRIGHT M, SMITH HK, MALINOWSKA M, SCHNABEL S, PAULO M-J, CATTONARO F, VENDRAMIN V, SCALABRIN S, JANZ D, DOUTHE C, BRENDEL O, BURE C, COHEN D, HUMMEL I, LE THIEC D, VAN EEUWIJK F, KEURENTJES J, FLEXAS J, MORGANTE M, ROBSON P, BOGEAT-TRIBOULOT M-B, TAYLOR G, POLLE A. 2017. Genes and gene clusters related to genotype and drought-induced variation in saccharification potential, lignin content, and wood anatomical traits in Populus nigra. Tree Physiology. doi:10.1093/treephys/tpx054
  • BALDACCHINI C, CASTANHEIRO A, MAGHAKYAN N, SGRIGNA G, VERHELST J, ALONSO R, AMORIM J, BELLAN P, BREUSTE J, BÜHLER O, CANTAR I, CARIÑANOS P, CARRIERO, G, DINCA L, GALINA C , ESPOSITO R, GAWRONSKI S, KERN M, LE THIEC D, MORETTI M, NINGAL T, RANTZOUDI E, SINJUR I, STOJANOVA B, ANICIC M, VELIKOVA V, ZIVOJINOVIC I, SAHAKYAN L, CALFAPIETRA C, SAMSON R. 2017. How does the amount and composition of PM deposit on Platanus acerifolia leaves change across different cities in Europe? Environmental Science & Technology. DOI: 10.1021/acs.est.6b04052
  • JOLIVET Y, BAGARD M, CABANE M, VAULTIER MN, GANDIN A, AFIF D, DIZENGREMEL P, LE THIEC D. 2016. Deciphering the ozone-induced changes in cellular processes: a prerequisite for ozone risk assessment at the tree and forest levels. Annals of Forest Sciences. 73(4), 923-943. DOI 10.1007/s13595-016-0580-3
  • CASTELL J-F, LE THIEC D. 2016. Impacts de l’ozone sur l’agriculture et les forêts et estimation des coûts économiques. Pollution Atmosphérique. Climat, santé, société. N° spécial, septembre 2016. 128-138
  • GROTE R, SAMSON R, ALONSO R, AMORIM JU, CALFAPIETRA C, CARIÑANOS P, CHURKINA G, FARES D, LE THIEC D, NIINEMETS U, MIKKELSEN TN, PAOLETTI E, TIWARY A. 2016. Functional traits of urban trees in relation to their air pollution mitigation potential: a holistic discussion. Frontiers in Ecology and Environment. doi:10.1002/fee.1426. IF 8,504
  • BAGARD M, JOLIVET Y, HASENFRATZ-SAUDER, MP, GERARD J, DIZENGREMEL P, LE THIEC D. 2015. Ozone exposure and flux-based response functions for photosynthetic traits in wheat, maize and poplar Environmental Pollution 206 : 411-420.
  • BÜKER P, FENG Z, UDDLING J, BRIOLAT A, ALONSO R, BRAUN S, ELVIRA S, GEROSA G, KARLSSON PE, LE THIEC D, MARZUOLI R, MILLS G, OKSANEN E, WIESER G, WILKINSON M, EMBERSON LD. 2015. New flux based dose-response relationships for ozone for European forest tree species. Environmental Pollution 206 : 163-174. http://dx.doi.org/10.1016/j.envpol.2015.06.033
  • RASHEED F, DREYER E, RICHARD B, BRIGNOLAS F, BRENDEL O, LE THIEC D. 2015. Vapour pressure deficit during growth has little impact on genotypic differences of transpiration efficiency at leaf and whole-plant level: an example from Populus nigra L. Plant, Cell & Environment 38 : 670-684
  • HEINEN R, BIENERT GP, COHEN D, CHEVALIER AS, UEHLEIN N, HACHEZ C, KALDENHOFF R, LE THIEC D, CHAUMONT F. 2014. Expression and characterization of plasma membrane aquaporins in stomatal complexes of Zea mays. Plant Molecular Biology 86 : 335-350. DOI 10.1007/s11103-014-0232-7
  • DUMONT J, KESKI-SAARI S, KEINÄNEN M, COHEN D, NINGRE N, KONTUNEN-SOPPELA S, BALDET P, GIBON Y DIZENGREMEL P, VAULTIER MN, JOLIVET Y, OKSANEN E, LE THIEC D. 2014. Ozone effects on ascorbate and glutathione biosynthesis pathways in relation with amino acid contents on three poplar genotypes. Tree Physiology. 34 : 253-266.
  • PANTIN F, RENAUD J, BARBIER F, VAVASSEUR A, LE THIEC D, ROSE C, BARIAC T, CASSON S, MCLACHLAN D, HETHERINGTON AM, MULLER B, SIMONNEAU T. 2013. Developmental priming of stomatal sensitivity to abscisic acid by leaf microclimate. Current Biology. 23 : 1805-1811
  • SCHLUCK N, ROSE C, LE THIEC D, BOULANGER K. 2013. Un fragment de toile en coton découvert dans le frigidarium d'une villa gallo-romaine à Damblain (Vosges, France). Archaeological Textiles Review. 54 : 52-56
  • DUMONT J, SPICHER F, MONTPIED P, DIZENGREMEL P, JOLIVET Y, LE THIEC D. 2013. Effects of ozone on stomatal responses to environmental parameters (blue light, red light, CO2 and vapour pressure deficit) in three Populus deltoides x Populus nigra genotypes. Environmental Pollution. 173 : 85-96
  • DGHIM AA, DUMONT J, HASENFRATZ-SAUDER MP, DIZENGREMEL P, LE THIEC D, JOLIVET Y. 2013. Capacity for NADPH regeneration in the leaves of two poplar genotypes differing in ozone sensitivity. Physiologia Plantarum 148 : 36-50
  • DGHIM AA, MHAMDI A, VAULTIER MN, HASENFRATZ-SAUDER MP, LE THIEC D, DIZENGREMEL P, NOCTOR G, JOLIVET Y. 2013. Analysis of cytosolic isocitrate dehydrogenase and glutathione reductase 1 in photoperiod-influenced responses to ozone using Arabidopsis knockout mutants. Plant, Cell & Environment 36 : 1981-1991
  • DIZENGREMEL P, VAULTIER MN, LE THIEC D, CABANÉ M, BAGARD M, GÉRANT D, GÉRARD J, DGHIM AA, RICHET N, PIREAUX JC, HASENFRATZ-SAUDER MP, JOLIVET Y. 2012. Phosphoenolpyruvate plays a prominent role in plant carbon metabolism – the case of cells demanding to survive ozone stress. New Phytologist 195 : 512-517.
  • RASHEED F, DREYER E, RICHARD B, BRIGNOLAS F, MONTPIED P, LE THIEC D. 2013. Genotype differences in 13C discrimination between atmosphere and leaf matter match differences in transpiration efficiency at leaf and whole-plant levels in hybrid Populus deltoides x nigra. Plant, Cell & Environment 36 : 87-102.
  • MATYSSEK R, WIESER G, CALFAPIETRA C, DE VRIES W, DIZENGREMEL P, ERNST D, JOLIVET Y, MIKKELSEN TN, MOHREN GMJ, LE THIEC D, TUOVINEN JP, WEATHERALL A, PAOLETTI E. 2012. Forests under climate change and air pollution: gaps in understanding and future directions for research. Environmental Pollution 160 : 57-65
  • RASHEED F, RICHARD B, LE THIEC D, MONTPIED P, PAILLASSA E, BRIGNOLAS F, DREYER E. 2011. Time course of delta13C in poplar wood: genotype ranking remains stable over the life-cycle in plantations despite some differences between cellulose and bulk-wood. Tree Physiology 31 : 1183-1193.
  • Bonal D, Ponton S, Le Thiec D, Richard B, Ningre N, Hérault B, Gonzalez S, Pignal M, Simonis E, Sabatier D, Guehl JM. 2011. Increasing water-use efficiency in two northern Amazonian tree species over the last two centuries: an historical 13C study using herbarium samples. Plant, Cell & Environment : 34 : 1332-1344.
  • FICHOT R, CHAMAILLARD S, DEPARDIEU C, LE THIEC D, COCHARD H, BARIGAH TS BRIGNOLAS F. 2011. Hydraulic efficiency and coordination with xylem resistance, leaf function and growth performance among eight unrelated Populus deltoides × Populus nigra hybrids. Journal of Experimental Botany 62 : 2093-2106.
  • Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N. 2010. The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance. Plant Journal 64 : 563-576
  • Cohen D, Bogeat-Triboulot M-B, Tisserant E, Balzergue S, Martin-Magniette M-L, Lelandais G, Ningre N, Renou J-P, Tamby J-P, Le Thiec D, Hummel I.2010. Comparative transcriptomics of drought responses in Populus: a meta-analysis of genome-wide expression profiling in mature leaves and root apices across two genotypes. Accepté à BMC Genomics
  • FICHOT R, BARIGAH TS, CHAMAILLARD S, LE THIEC D, LAURANS F, COCHARD H, BRIGNOLAS F. 2010. Common trade-offs between xylem resistance to cavitation and other physiological traits do not hold among unrelated Populus deltoides x Populus nigra hybrids. Plant, Cell and Environment 33 : 1553-1568
  • Rineau F, Rose C, Le ThiecD, Garbaye J. 2010. The storage of mineral elements in the mantle of Lactarius subdulcis-beech ectomycorrhizas is increased by liming. Fungal Biology 114 : 1007-1014
  • Gourcilleau D, Bogeat-Triboulot MB, Le ThiecD, Lafon-Placette C, Delauney A, El-Soud WA, Brignolas F, Maury S. 2010. DNA methylation and histone acetylation: genetic variations in hybrid poplars, impact of water deficit and relationships with productivity. Annals of Forest Science. 67, doiI: 10.1051/forest/2009101
  • Dizengremel P, Le Thiec D, Hasenfratz-SauderMP, Vaultier MN, Bagard M, Jolivet Y. 2009. Effective ozone dose: implication of metabolic-dependent changes in redox power. Plant Biology 11 : 35-42.
  • Roussel M, Le Thiec D, Montpied P, Guehl JM, Brendel O. 2009. Diversity of water use efficiency in a Quercus robur family: contribution of related leaf traits. Annals of Forest Science 66 : 408-417.
  • BagardM, Le ThiecD, DelacôteE, Hasenfratz-SauderMP, BanvoyJ, Gérard J, DizengremelP, Jolivet Y. 2008. Ozone-induced changes in photosynthesis and photorespiration of hybrid poplarin relation to the developmental stage of the leaves. Physiologia Plantarum 134 : 559-574..
  • Dizengremel P, Le Thiec D, Bagard M, Jolivet Y. 2008. Ozone risk assessment for plants: central role of metabolism-dependent changes in reducing power. Environmental Pollution 156 : 11-15.
  • Brendel O., Le Thiec D., Saintagne C., Kremer A., Guehl J.-M. 2008. Detection of quantitative trait loci controlling water use efficiency and related traits in Quercus robur L. Tree Genetics and Genomes : 4 : 263-278
  • Rinaldi C, Kohler A, Frey P, Duchaussoy F, Ningre N, Couloux A, Wincker P, Le Thiec D, Fluch S, Martin F, Duplessis S. 2007. Transcript profiling of poplar leaves upon infection with compatible and incompatible strains of the foliar rust Melampsora larici-populina.Plant Physiology 144 : 347-366.
  • Karlsson PE, Braun S, Broadmeadow M, Elvira S, Emberson L, Gimeno BS, Le Thiec D, Novak K, Oksanen E, Schaub M, Uddling J, Wilkinson M. 2007. Risk assessments for forest trees: The performance of the ozone flux versus the AOT concepts. Environmental Pollution 146 : 608-616
  • Vansteenkiste D, Van Acker J, Stevens M, Le Thiec D, Nepveu G. 2007. Composition, distribution and supposed origin of mineral inclusions in sessile oak wood – consequences for microdensitometrical analysis. Annals of Forest Science, 64 : 11-19.
  • Leitao L, Delacôte E, Dizengremel P, Le Thiec D, Biolley JP. 2007. Assessment of the impact of increasing concentrations of ozone on photosynthetic components of maize (Zea mays L.), a C4 plant. Environmental Pollution, 146 : 5-8.
  • Bogeat-Triboulot M-B, Brosche M, Renaut J, Jouve L, Le Thiec D, Fayyaz P, Vinocur B, Witters E, Laukens K, Teichmann T, Altman A, Hausman J-F, Polle A, Kangasjarvi J, Dreyer E. 2007. Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology and growth performance in Populus euphratica, a poplar growing in arid regions. Plant Physiology 143 : 876-892.
  • Matyssek R, Le Thiec D, Löw M, Dizengremel P, Nunn AJ, Häberle KH. 2006. Interactions between drought and O3 stress in forest trees. Plant Biology 8 : 11-17
  • Monclus R, Dreyer E, Villar M, Delmotte FM, Delay D, Petit JM, Barbaroux C, Le Thiec D, Bréchet C, Brignolas F. 2006.Impact of drought on productivity and water use efficiency in 29 genotypes of Populus deltoides×Populus nigra.New Phytologist 169 : 765-777
  • Hukin D, Cochard H, Dreyer E, Le Thiec D, Bogeat-Triboulot MB. 2005. Cavitation vulnerability in roots and shoots: does Populus euphratica Oliv., a poplar from arid areas of Central Asia, differ from other poplar species? Journal of Experimental Botany, 56 : 2003-2010..
  • Karlsson PE, Uddling J, Braun S, Broadmeadow M, Elvira S, Gimeno BS, Le Thiec D, Oksanen E, Vandermeiren K, Wilkinson M, Emberson L. 2004. New critical levels for ozone effects on young trees based on AOT40 and simulated cumulative leaf uptake of ozone. Atmospheric Environment, 38, 2283-2294.
  • Le Thiec D, Manninen S. 2003. Ozone and water deficit reduced growth of Aleppo pine seedlings. Plant Physiology and Biochemistry, 41, 55-63.
  • Dalstein L, Torti X, Le Thiec D, Dizengremel P. 2002. Physiological study of declining Pinus cembra (L.) trees in southern France. Trees, Structure and Function, 16 : 299-305.
  • Kytoviita MM, Le Thiec D, Dizengremel P. 2001. Elevated CO2 and ozone reduce the nitrogen acquisition by Pinus halepensis from its mycorrhizal symbiont. Physiologia Plantarum, 111, 303-312.
  • Manninen S, Le Thiec D, Rose C, Nourrisson G, Radnai F, Garrec JP, Huttunen S. 1999. Pigment concentrations and ratios of Aleppo pine seedlings exposed to ozone. Water, Air, and Soil Pollution 116 : 333-338
  • Dixon M, Le Thiec D, Garrec JP. 1998. Reactions of Picea abies and Fagus sylvatica trees to two years of ozone exposure and episodic drought. Environmental and Experimental Botany 40: 77-91
  • Dixon M, Le Thiec D, Garrec JP. 1997. An investigation into the effects of ozone and drought, applied singly and in combination, on the quantity and quality of the epicuticular wax of Norway spruce. Plant Physiology and Biochemistry 35: 447-454
  • Le Thiec D, Dixon M. 1996. Acclimation of photosynthesis in Norway spruce and red oak grown in open-top chambers and subjected to natural drought and to elevated CO2. Canadian Journal of Forest Research 26: 87-94.).
  • Le Thiec D, Dixon M, Garrec JP. 1995. Seasonal and annual variations of phosphorus, calcium, potassium and manganese contents in different cross-sections of Picea abies (L.) Karst. needles and Quercus rubra (L.) leaves exposed to elevated CO2. Trees 10: 55-62
  • Le Thiec D, Dixon M, Garrec JP. 1995. Seasonal and annual variations of potassium and calcium contents in different cross-sections of Picea abies (L.) Karst. needles and Fagus sylvatica (L.) leaves exposed to ozone and mild water stress. Annales des Sciences Forestières 52: 411-422..
  • Dixon M, Le Thiec D, Garrec JP. 1995. The growth and gas exchange response of soil planted Norway spruce (Picea abies L. Karst.) and red oak (Quercus rubra L.) exposed to elevated CO2 and to naturally occurring drought stress. New Phytologist 129: 265-273
  • Le Thiec D, Dixon M, Garrec JP. 1994. The effects of slightly elevated ozone concentrations and mild drought stress on the physiology and growth of Norway spruce, Picea abies (L.) Karst. and beech, Fagus sylvatica (L.), in open-top chambers. New Phytologist 128: 671-678.
  • Le Thiec D, Rose C, Garrec JP, Laffray D, Louguet P, Galaup S, Loosveldt P. 1994. Alteration of element contents in guard cells of Norway spruce (Picea abies) subjected to ozone fumigation and (or) water stress: X-ray microanalysis study. Canadian Journal of Botany 72: 86-92.

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